Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

Abstract In this study, an analysis method was established for the quantification of residues halquinol and its metabolites in livestock fishery products using liquid chromatography–tandem mass spectrometry (LC–MS/MS). We selected beef, pork (muscle fat), chicken, egg, milk, flat fish, eel, shrimp as target samples validation owing to them being typical products. Validation developed performed (LC–MS/MS) at three concentration levels (0.5, 1, 2 × maximum residue limits) following Codex Alimentarius (CODEX) guidelines (CAC/GL 71–2009). For all samples, correlation coefficients (R ) exceeded 0.99, recoveries ranged between 75.59 119.36%, variation (CV) 1.39 28.66%, thus satisfying CODEX guidelines. addition, inter-laboratory conducted, resulting CVs satisfied guidelines; LOQ 10 μg kg –1 pig muscle 5 other samples. Therefore, study can accurately precisely screen quantify